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cn01  (Cytoskeleton Inc)
95
Cytoskeleton Inc cn01
Fig. 1. ROCK inhibition decreases while Rho activation increases Young's modulus of preadipocytes and differentiated adipocytes. (A) AFM probe approaching a preadipocyte. Arrow: nucleus; arrowhead: cytoplasm. Representative force-displacement curves of preadipocytes when probing on nucleus (B) and cytoplasm (C). ROCK inhibition and Rho activation were conducted using Y-27632 and <t>CN01,</t> respectively. (D) AFM probing on differentiated adipocytes. Arrow: nucleus; arrowhead: lipid droplet. Representative force- displacement curves of differentiated adipocytes when probing on nucleus (E) and lipid droplet (F). For both preadipocytes (G) and differentiated adipocytes (H), ROCK inhibi- tion by Y-27632 decreased while Rho activation by CN01 increased cellular Young's modulus. *: p < 0.05, **: p < 0.01, and ***: p < 0.005 based on repeated AFM probing (n numbers). Scale bar: 20 mm.
Cn01, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 1. ROCK inhibition decreases while Rho activation increases Young's modulus of preadipocytes and differentiated adipocytes. (A) AFM probe approaching a preadipocyte. Arrow: nucleus; arrowhead: cytoplasm. Representative force-displacement curves of preadipocytes when probing on nucleus (B) and cytoplasm (C). ROCK inhibition and Rho activation were conducted using Y-27632 and CN01, respectively. (D) AFM probing on differentiated adipocytes. Arrow: nucleus; arrowhead: lipid droplet. Representative force- displacement curves of differentiated adipocytes when probing on nucleus (E) and lipid droplet (F). For both preadipocytes (G) and differentiated adipocytes (H), ROCK inhibi- tion by Y-27632 decreased while Rho activation by CN01 increased cellular Young's modulus. *: p < 0.05, **: p < 0.01, and ***: p < 0.005 based on repeated AFM probing (n numbers). Scale bar: 20 mm.

Journal: Biochemical and biophysical research communications

Article Title: Rho/ROCK mechanosensor in adipocyte stiffness and traction force generation.

doi: 10.1016/j.bbrc.2022.03.078

Figure Lengend Snippet: Fig. 1. ROCK inhibition decreases while Rho activation increases Young's modulus of preadipocytes and differentiated adipocytes. (A) AFM probe approaching a preadipocyte. Arrow: nucleus; arrowhead: cytoplasm. Representative force-displacement curves of preadipocytes when probing on nucleus (B) and cytoplasm (C). ROCK inhibition and Rho activation were conducted using Y-27632 and CN01, respectively. (D) AFM probing on differentiated adipocytes. Arrow: nucleus; arrowhead: lipid droplet. Representative force- displacement curves of differentiated adipocytes when probing on nucleus (E) and lipid droplet (F). For both preadipocytes (G) and differentiated adipocytes (H), ROCK inhibi- tion by Y-27632 decreased while Rho activation by CN01 increased cellular Young's modulus. *: p < 0.05, **: p < 0.01, and ***: p < 0.005 based on repeated AFM probing (n numbers). Scale bar: 20 mm.

Article Snippet: ForRho activation, CN01 (Cytoskeleton Inc.)wasdissolved into 20ml dimethyl sulfoxideanddiluted inDMEM at a concentration of 1 unit/ml.

Techniques: Inhibition, Activation Assay

Fig. 2. ROCK inhibitor (Y-27632) disrupts and Rho activator (CN01) strengthens stressed actin filament formation in adipose cells. Actin was immunostained with rhodamine phalloidin and nuclei with DAPI. Scale bar: 20 mm.

Journal: Biochemical and biophysical research communications

Article Title: Rho/ROCK mechanosensor in adipocyte stiffness and traction force generation.

doi: 10.1016/j.bbrc.2022.03.078

Figure Lengend Snippet: Fig. 2. ROCK inhibitor (Y-27632) disrupts and Rho activator (CN01) strengthens stressed actin filament formation in adipose cells. Actin was immunostained with rhodamine phalloidin and nuclei with DAPI. Scale bar: 20 mm.

Article Snippet: ForRho activation, CN01 (Cytoskeleton Inc.)wasdissolved into 20ml dimethyl sulfoxideanddiluted inDMEM at a concentration of 1 unit/ml.

Techniques:

Fig. 3. Traction forces in adipose cells are decreased by ROCK inhibition, and traction force dependency on cell spreading area is increased by Rho activation. (AeC) Representative TFM images of preadipocytes from control, Y-27632 treatment, and CN01 treatment, respectively. (D) Histogram of traction forces from TFM traction force vectors measured for preadipocytes. (E) Average traction force for each cell obtained from entire traction force vectors. ROCK inhibition by Y-27632 significantly decreased traction force in preadipocytes, while Rho activation by CN01 did not induce significant change. (FeH) Representative TFM images of differentiated adipocytes. (I) Traction force histogram for differentiated adipocytes. (J) Differentiated adipocytes displayed substantially lower traction forces than preadipocytes. Trends of Y-27632 and CN01 exposure on the traction force of differ- entiated adipocytes were similar to those in preadipocytes. (K,L) Traction force dependency on projected cell spreading area is increased by Rho activation, and such dependency is less distinct in differentiated adipocytes. **: p < 0.01, ***: p < 0.001, and n.s.: non-significant. Scale bar: 5 mm.

Journal: Biochemical and biophysical research communications

Article Title: Rho/ROCK mechanosensor in adipocyte stiffness and traction force generation.

doi: 10.1016/j.bbrc.2022.03.078

Figure Lengend Snippet: Fig. 3. Traction forces in adipose cells are decreased by ROCK inhibition, and traction force dependency on cell spreading area is increased by Rho activation. (AeC) Representative TFM images of preadipocytes from control, Y-27632 treatment, and CN01 treatment, respectively. (D) Histogram of traction forces from TFM traction force vectors measured for preadipocytes. (E) Average traction force for each cell obtained from entire traction force vectors. ROCK inhibition by Y-27632 significantly decreased traction force in preadipocytes, while Rho activation by CN01 did not induce significant change. (FeH) Representative TFM images of differentiated adipocytes. (I) Traction force histogram for differentiated adipocytes. (J) Differentiated adipocytes displayed substantially lower traction forces than preadipocytes. Trends of Y-27632 and CN01 exposure on the traction force of differ- entiated adipocytes were similar to those in preadipocytes. (K,L) Traction force dependency on projected cell spreading area is increased by Rho activation, and such dependency is less distinct in differentiated adipocytes. **: p < 0.01, ***: p < 0.001, and n.s.: non-significant. Scale bar: 5 mm.

Article Snippet: ForRho activation, CN01 (Cytoskeleton Inc.)wasdissolved into 20ml dimethyl sulfoxideanddiluted inDMEM at a concentration of 1 unit/ml.

Techniques: Inhibition, Activation Assay, Control